ANTI-FLAG® M1 Agarose Affinity Gel
SIGMA/A4596
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| antibody product type | primary antibodies |
| capacity | ≥0.6 mg/mL, gel binding capacity |
| conjugate | agarose conjugate |
| form | suspension |
| isotype | IgG12b |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins. Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins. immunoprecipitation (IP): suitable Elution - FLAG peptide, Glycine, pH 3.5 EDTA Learn more product details in our FLAG® application portal. |
| Features and Benefits: | • Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step. • Fusion protein may be eluted from affinity resin by mild elution with EDTA. • A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins. |
| General description: | ANTI-FLAG® M1 Agarose Affinity Gel is a purified mouse IgG2B monoclonal antibody covalently attached to agarose. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins. |
| Physical form: | Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide |
| Specificity: | Binding specificity: Free N-Terminus of FLAG sequence N-Asp-Tyr-Lys-Asp-Asp-Asp |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |