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Anti-iASPP antibody, Mouse monoclonal

SIGMA/A4605 - clone LXO49.3, purified from hybridoma cell culture

Synonym: Anti-Inhibitory Member of the Apoptosis-stimulating Protein of p53 Family; Anti-RAI; Anti-Rel-associated Inhibitor

MDL Number: MFCD08705587
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A4605-100UL 100 µL
$635.00
1/EA
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Immunoblotting
Osteogenic sarcoma (Human bone) (U-2-OS) cell extract was separated on SDS-PAGE and probed with Monoclonal PA to IASPP (MO) CL: LXO 49.3 (Cat. No. A4605). The antibody was developed with HRP APA Mouse Fab ads/HuBovHor (gt) (Cat. No. A2304) and ECL substrate.
Antibody concentration 0.2 μg/mL.

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone LXO49.3, monoclonal
concentration ~2 mg/mL
conjugate unconjugated
form buffered aqueous solution
isotype IgG1
mol wt antigen ~100 kDa
Quality Level 200 
shipped in dry ice
species reactivity mouse, human
storage temp. −20°C
target post-translational modification unmodified
technique(s) immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)
UniProt accession no. Q8WUF5 
Application: Monoclonal Anti-iASPP antibody produced in mouse has been used in:
• immunoblotting
• immunocytochemistry
• immunoprecipitation

Application: Mouse monoclonal Anti-iASPP antibody can be used for western blot (0.1-0.2μg/mL using total cell extract of human osteogenic sarcoma), microarray, immunoprecipitation and immunocytochemistry.
Biochem/physiol Actions: PPP1R13L (protein phosphatase 1 regulatory subunit 13 like)/iASPP inhibits p53-mediated cell death. It interacts and inhibits NFkB p65 RelA. It may act as a therapeutic target for bladder cancer (BC). It modulates cell proliferation and apoptosis. iASPP plays a key role in breast cancer and acute leukemia.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: iASPP is an inhibitory member of the apoptosis-stimulating protein of p53 (ASPP) family. It is an oncoprotein that associates with Ras, E1A and E7 to transform cells.
General description: Monoclonal Anti-iASPP (mouse IgG1 isotype) is derived from the hybridoma LXO49, produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding residues of human iASPP. The ASPP family of proteins contains three members, ASSP1, ASSP2 and iASPP. iASPP is also known as REL associated inhibitor (RAI). iASPP exists as a 100 kDa form and as a 70 kDa form (iASPP/RAI). It is found both in the nucleus and cytoplasm, while iASPP/RAI is mainly cytosolic. PPP1R13L (protein phosphatase 1 regulatory subunit 13 like) codes for iASPP, that is located on human chromosome 19q13.
Physical form: Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Hazard Codes Xn
Risk Statements 21/22
Safety Statements 36/37
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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