Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
SIGMA/A4656 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (whole molecule)−AP
MDL Number: MFCD00162640
Product Type: Chemical
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | human |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:1,000 |
| Application: | Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays. |
| Application: | Immunoprecipitated 293T cells lysates were analyzed by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at 1:7500. |
| Application: | Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody binds to mouse IgGs and does not cross-react with human serum proteins. |
| Immunogen: | purified mouse IgG |
| Other Notes: | Antibody adsorbed with human serum proteins. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |