Monoclonal Anti-Actin antibody produced in mouse
SIGMA/A4700 - clone AC-40, ascites fluid
Synonym: Monoclonal Anti-Actin
Product Type: Chemical
Western Blotting JOURNAL CITATION: A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. By: Kinnaird, J. H., Weir, W., et al. in PLoS One, 2013. PubMed ID: 23840536 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0066833), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells. By: Wang, C., Yu, J., et al. in PLoS One, 2008. PubMed ID: 18949048 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0003523), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Development of an oligonucleotide-based fluorescence assay for the identification of tyrosyl-DNA phosphodiesterase 1 (TDP1) inhibitors. By: Walker, S., Meisenberg, C., et al. in Anal Biochem, 2014. PubMed ID: 24637157 Image collected and cropped by CiteAb from the following publication, (http://linkinghub.elsevier.com/retrieve/pii/S0003269714000931), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Tissue stained with conserved C-terminus specific Monoclonal Anti-Actin (Cat. No. A4700). Indirect immunofluorescence staining of human tongue frozen section with Monoclonal Anti-Actin, Clone No. AC-40 (Cat. No. A4700).
Tissue stained with conserved C-terminus specific Monoclonal Anti-Actin (Cat. No. A4700). Indirect immunofluorescence staining of human tongue frozen section with Monoclonal Anti-Actin, Clone No. AC-40 (Cat. No. A4700).
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A4700). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40 (Cat. No. A4700). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat (Cat. No. F5262). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A4700). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC-40, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable using methanol-fixed, frozen sections |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:200 using cultured human or chicken fibroblasts | |
| western blot: 1:500 using cultured human or chicken fibroblast extract |
| Application: | Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. |
| Application: | Monoclonal rabbit anti-actin has been used: • in the detection of actin in HeLa cells, breast cancer cell line and leukemia cell line using western blotting • in human fibroblasts by immunofluorescence detection |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Among the six isoforms of actin, four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (skeletal, cardiac, and smooth muscle), one β−actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. The actin in cells of various species and tissues are very similar in their immunological and physical properties characterized by electrophoresis and amino acid sequence analysis.(from bulletin) They mediate cytoskeletal remodeling during T cell activation. Mutation in the γ-actin and β-actin gene is associated with a developmental disorder called Baraitser-Winter syndrome. Mutation in the γ-actin leads to hearing loss. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin and myosin are major cytoskeletal proteins. Actin is expressed majorly in six isoforms. The human γ-actin gene is mapped to human chromosome 17. The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. A synthetic actin C-terminal peptide attached to Multiple Antigen Peptide (MAP) backbone was used as the immunogen. The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog No. ISO2. |
| General description: | The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. |
| General description: | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. |
| Immunogen: | synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Supplied as ascites fluid with 15mM sodium azide as a preservative. |
| Specificity: | Monoclonal Anti-Actin recognizes an epitope located on the C-terminal end of actin, but not on the N-terminal end. This epitope is conserved in all actin isoforms. The antibody specifically labels actin in a wide variety of tissues and species in immunoblotting (band at 42 kDa), in immunofluorescent staining of cultured cell lines and in immunohistology. Cross-reaction has been observed with human, bovine, sheep, goat, pig, rabbit, dog, mouse, rat, guinea pig, hamster, chicken, carp, viper, frog, and snail. The antibody may be used in staining of methanol-fixed, frozen sections, and the epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solution may also be used as fixatives. |
| Storage and Stability: | For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |