Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse
SIGMA/A4721 - ~2 mg/mL, clone CYT2-21, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03454755
Product Type: Chemical
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in Mouse, Clone: CYT2-21 (Cat. No. A4721). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and 1:10,000 ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636). Lanes 1. 5 μg/mL antibody 2. 10 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CYT2-21, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 40 kDa |
| Quality Level | 200  |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 5-10 μg/mL using a whole extract of rat brain | |
| UniProt accession no. | Q99418  |
| Application: | Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications. |
| Application: | To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas. Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO. |
| Immunogen: | recombinant, human ARNO (ARFnucleotide-binding-si |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |