Asialofetuin from fetal calf serum
SIGMA/A4781 - Type I (Sigma designation)
MDL Number: MFCD00130561
Product Type: Chemical
biological source | bovine (calf) serum |
form | powder |
impurities | salt, essentially free |
≤0.5% N-acetylneuraminic acid | |
Quality Level | 300 |
solubility | 0.85% sodium chloride: soluble 1 mg/mL |
storage temp. | 2-8°C |
type | Type I (Sigma designation) |
Application: | Asialofetuin from fetal calf serum has been used: • to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA) • quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA • as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins • to study the nature of the interaction between ferritin and the placenta |
Application: | Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy. |
Biochem/physiol Actions: | Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R. |
General description: | Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues. |
Packaging: | 1 g in poly bottle |
Packaging: | 50, 250 mg in poly bottle |
Preparation Note: | Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960). |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |