Asialofetuin from fetal calf serum
SIGMA/A4781 - Type I (Sigma designation)
MDL Number: MFCD00130561
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A4781-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A4781-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A4781-50MG
| biological source | bovine (calf) serum |
| form | powder |
| impurities | salt, essentially free |
| ≤0.5% N-acetylneuraminic acid | |
| Quality Level | 300  |
| solubility | 0.85% sodium chloride: soluble 1 mg/mL |
| storage temp. | 2-8°C |
| type | Type I (Sigma designation) |
| Application: | Asialofetuin from fetal calf serum has been used: • to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA) • quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA • as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins • to study the nature of the interaction between ferritin and the placenta |
| Application: | Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy. |
| Biochem/physiol Actions: | Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R. |
| General description: | Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 50, 250 mg in poly bottle |
| Preparation Note: | Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |