N,N′-Bis(acryloyl)cystamine
SIGMA/A4929 - BioReagent, suitable for electrophoresis
Synonym: 2,2′-(Bisacrylamino)diethyl disulfide; BAC; Bis(2-
CAS Number: 60984-57-8
Empirical Formula (Hill Notation): C10H16N2O2S2
Molecular Weight: 260.38
EC Number: 262-546-8
MDL Number: MFCD00036225
Linear Formula: C10H16N2O2S2
Product Type: Chemical
form | powder |
InChI | 1S/C10H16N2O2S2/c1-3-9(13 |
InChI key | DJVKJGIZQFBFGS-UHFFFAOYSA |
product line | BioReagent |
Quality Level | 200 |
SMILES string | C=CC(=O)NCCSSCCNC(=O)C=C |
storage temp. | −20°C |
suitability | suitable for electrophoresis |
Application: | BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. |
Application: | BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel. |
Packaging: | 1, 5 g in glass bottle |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352116 |