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N,N′-Bis(acryloyl)cystamine

SIGMA/A4929 - BioReagent, suitable for electrophoresis

Synonym: 2,2′-(Bisacrylamino)diethyl disulfide; BAC; Bis(2-acrylamidoethyl) disulfide

CAS Number: 60984-57-8
Empirical Formula (Hill Notation): C10H16N2O2S2
Molecular Weight: 260.38
EC Number: 262-546-8
MDL Number: MFCD00036225
Linear Formula: C10H16N2O2S2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-A4929-5G 5 g
 $578.00
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form powder
InChI 1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)
InChI key DJVKJGIZQFBFGS-UHFFFAOYSA-N
product line BioReagent
Quality Level 200 
SMILES string C=CC(=O)NCCSSCCNC(=O)C=C
storage temp. −20°C
suitability suitable for electrophoresis
Application: BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
Application: BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
Packaging: 1, 5 g in glass bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352116

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