Adenosine Deaminase from bovine spleen
SIGMA/A5043 - Type X, buffered aqueous glycerol solution, ≥130 units/mg protein
Synonym: ADA; adenosine aminohydrolase; Adenosine aminohydrolase
CAS Number: 9026-93-1
MDL Number: MFCD00081287
Product Type: Chemical
| biological source | bovine spleen |
| cation traces | NH4+: ≤10 μg/mg protein |
| foreign activity | alkaline phosphatase ≤0.01% |
| guanase ≤0.01% | |
| nucleoside phosphorylase ≤0.1% | |
| form | buffered aqueous glycerol solution |
| mol wt | 32.5-33 kDa |
| Quality Level | 200  |
| specific activity | ≥130 units/mg protein |
| storage temp. | 2-8°C |
| type | Type X |
| UniProt accession no. | P56658  |
| Analysis Note: | Protein determined by biuret. |
| Application: | Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies. |
| Application: | Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease. |
| Biochem/physiol Actions: | Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease. |
| General description: | Molecular weight: 32.5-33 kDa pI: 4.85 |
| Physical form: | Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0 |
| Unit Definition: | One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥130 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.4.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |