Monoclonal Anti-α-Actinin antibody produced in mouse
SIGMA/A5044 - clone BM-75.2, ascites fluid
Synonym: Anti-BDPLT15
MDL Number: MFCD00162107
Product Type: Chemical
Western Blotting JOURNAL CITATION: mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4. By: Castets, P., Rion, N., et al. in Nat Commun, 2019. PubMed ID: 31320633 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-019-11227-4), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Alternative splicing regulates vesicular trafficking genes in cardiomyocytes during postnatal heart development. By: Giudice, J., Xia, Z., et al. in Nat Commun, 2014. PubMed ID: 24752171 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms4603), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Double labeling of mouse aorta cells using Monoclonal Anti-α-Smooth Muscle Actin, Clone No. 1A4, (Cat. No. A2547) and Monoclonal Anti-α-Actinin, Clone No. BM-75.2 (Cat. No. A5044).
From E. Ehler and Dr. A. Draeger, Institute of Molecular Biology, Academy of Sciences, Salzburg.
From E. Ehler and Dr. A. Draeger, Institute of Molecular Biology, Academy of Sciences, Salzburg.
Immunofluorescence
Localization of microfilament associated proteins in PTK2 cells using (1) Monoclonal Anti-Tropomyosin, Clone No. TM311 (Cat. No. T2780), (2) Monoclonal Anti-Myosin (Light Chain), Clone No. MY-21 (Cat. No. M4401) and (3) Monoclonal Anti-α-Actinin, Clone No. BM-75.2 (Cat. No. A5044).
From D. Girard, CHUL, Laval Univ., Sainte-Foy, Quebec.
Localization of microfilament associated proteins in PTK2 cells using (1) Monoclonal Anti-Tropomyosin, Clone No. TM311 (Cat. No. T2780), (2) Monoclonal Anti-Myosin (Light Chain), Clone No. MY-21 (Cat. No. M4401) and (3) Monoclonal Anti-α-Actinin, Clone No. BM-75.2 (Cat. No. A5044).
From D. Girard, CHUL, Laval Univ., Sainte-Foy, Quebec.
Immunofluorescence
Double labeling of Mouse aorta cells using Monoclonal Anti-α-Smooth Muscle Actin, Clone No. 1A4 (Cat. No. A2547) and Monoclonal Anti-α-Actinin, Clone No. BM-75.2 (Cat. No. A5044) with FITC-Goat Anti-Mouse IgM (μ-chain) (Cat. No. F9259) and TRITC-Anti-Mouse IgG.
From E. Ehler and Dr. A. Draeger, Institute of Molecular Biology, Academy of Sciences, Salzburg.
Double labeling of Mouse aorta cells using Monoclonal Anti-α-Smooth Muscle Actin, Clone No. 1A4 (Cat. No. A2547) and Monoclonal Anti-α-Actinin, Clone No. BM-75.2 (Cat. No. A5044) with FITC-Goat Anti-Mouse IgM (μ-chain) (Cat. No. F9259) and TRITC-Anti-Mouse IgG.
From E. Ehler and Dr. A. Draeger, Institute of Molecular Biology, Academy of Sciences, Salzburg.
Immunofluorescence Staining of an acetone-fixed section of human myocardium using Monoclonal Anti-α-Actinin (clone no. BM-75.2, Cat. No. A5044). The picture shows the distribution of non-sarcomeric α-actinin in endothelial and smooth muscle cells of two blood vessels, whereas there is no fluorescence in the surrounding cardio-myocytes. The nuclei are stained red with propidium iodide. From Prof. Dr. J. Schaper and Dr. S. Hein, Max Planck Institute, Bad Nauheim, Germany.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BM-75.2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, bovine, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:200 using cultured chicken fibroblasts |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P12814  |
| Application: | Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000. |
| Application: | May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin. |
| Application: | Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays. It can also be used in western blot, microarray, immunohistochemistry and in Immunofluorescence microscopy. |
| Biochem/physiol Actions: | α−actinin regulates muscle contraction and cytoskeletal organization. It promotes cell migration and acts as an actin crosslinker. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells. |
| General description: | The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily. |
| Immunogen: | cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |