Anti-Actin (20-33) antibody produced in rabbit
SIGMA/A5060 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-ACTA
MDL Number: MFCD01092261
Product Type: Chemical
Western Blotting JOURNAL CITATION: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis. By: Knopfová, L., Beneš, P., et al. in Mol Cancer, 2012. PubMed ID: 22439866 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis. By: Knopfová, L., Beneš, P., et al. in Mol Cancer, 2012. PubMed ID: 22439866 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis. By: Knopfová, L., Beneš, P., et al. in Mol Cancer, 2012. PubMed ID: 22439866 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry
Staining of formalin-fixed, paraffin-embedded section of rat tongue using Rabbit Anti-Actin (Cat. No. A5060).
Antibody dilution 1:100
Specific staining of actin.
Staining of formalin-fixed, paraffin-embedded section of rat tongue using Rabbit Anti-Actin (Cat. No. A5060).
Antibody dilution 1:100
Specific staining of actin.
Immunoblotting
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Rabbit Anti-Actin (Cat. No. A5060). The antibody was developed with Goat Anti-Rabbit IgG, Alkaline Phosphatase conjugate (Cat. No. A9919) and NBT/BCIP substrate.
Lanes
A: Antibody dilution 1:500
B: Negative control (only secondary antibody)
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Rabbit Anti-Actin (Cat. No. A5060). The antibody was developed with Goat Anti-Rabbit IgG, Alkaline Phosphatase conjugate (Cat. No. A9919) and NBT/BCIP substrate.
Lanes
A: Antibody dilution 1:500
B: Negative control (only secondary antibody)
Enhanced Validation-By Independent Antibodies Immunofluorescence. CFB cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:40 Anti-Actin antibody produced in Rabbit (Cat. No. A2066) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887), and 1:200 Anti-Actin (20-33) antibody produced in Rabbit (Cat. No. A5060) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887). Results Two Anti-Actin antibodies, A2066 (A) and A5060 (B), target different regions of Actin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Chicken intestine were stained with 1:100 Anti-Actin (20-33) antibody produced in Rabbit (Cat. No. A5060), followed by 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:aceton. Fixed cells were stained with 1:100 Rabbit Anti-Actin (Cat. No. A5060). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:250 Rabbit Anti-Actin (Cat. No. A5060). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. U87 3. COS7 4. P19 5. RAT2 6. CHO 7. MDBK 8. MDCK
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 42 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | wide range (animal), invertebrates |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using chicken or rat tissue sections |
| indirect immunofluorescence: 1:200 using cultured human fibroblasts | |
| microarray: suitable | |
| western blot: 1:250 using using rat brain or chicken muscle extracts |
| Application: | Homogenates from limb tissue of E13.5 mouse embryos were analyzed by western blot using rabbit anti-actin as a primary antibody at a dilution of 1:8000. |
| Application: | Rabbit Anti-Actin (20-33) antibody has been used for immunoblotting assays. The product can also be used for immunohistochemistry, indirect immunofluorescence and microarray analyses. |
| Biochem/physiol Actions: | Actin maintains the cell shape and participates in cytokinesis. Actin provides cellular strength and promotes active movement of the cell. Actin protein is essential for the formation of mature platelets. Upregulation of ACTA1 (actin, α 1) gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | A mature actin filament consists of a right-handed double helix made of two F-actin protofilaments. The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin. |
| General description: | Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). |
| Immunogen: | N-terminal actin peptide attached to a multiple antigen peptide (MAP) backbone. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative. |
| Specificity: | Anti-Actin recognizes an epitope located on the N-terminal region of actin. The antibody specifically labels actin in a wide variety of tissues and species using immunoblotting (band at 42kDa). It specifically stains typical stress fibers in cultured cells using indirect immunofluorescent staining and immunohistochemistry. |
| Storage and Stability: | For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |