Anti-Aurora B antibody produced in rabbit
SIGMA/A5102 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-AIM-1; Anti-AIR-2 Kinase; Anti-AIRK-2
MDL Number: MFCD02262515
Product Type: Chemical
Immunofluorescence HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit (Cat. No. A5102). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat (Cat. No. F9887).
Western Blotting Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit (Cat. No. A5102). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 41 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: 1:50 using HeLa cells | |
| microarray: suitable | |
| western blot: 1:1,000 using nuclei-enriched fraction of mouse NIH-3T3 cells | |
| western blot: 1:1,000 using using PC-12 rat phaeochromocytoma cells | |
| UniProt accession no. | Q96GD4  |
| Application: | Anti-Aurora B antibody has been used in: • in immunoblotting • in immunoprecipitation • in immunofluorescence • in immunofluorescence staining • in western blotting |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development. |
| Biochem/physiol Actions: | Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide. |
| Target description: | Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |