Anti-ABI1 antibody produced in rabbit
SIGMA/A5106 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Abelson interactor 1; Anti-Abl-interactor protein 1; Anti-Nap1 binding protein NAP1BP; Anti-eps8 binding protein; Anti-interactor protein AblBP4; Anti-spectrin SH3 domain binding protein 1 SSH3BP1
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-ABI1 antibody produced in Rabbit (Cat. No. A5106) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing ABI1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:3,000.
Immunoblotting Tissue extracts and cell lysates were separated on SDS-PAGE and blotted with Anti-Abi1 (Cat. No. A5106). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000. Lanes 1,6. rat brain 2. mouse brain 3. A431 4. HEK293T cells transfected with human Abi1 5. HEK293T cells untransfected 6. Inhibition with Abi1 immunizing peptide (human, 163-180) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~65 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, canine (predicted), mouse (predicted), rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using A431 cell lysate |
| western blot: 0.5-1.0 μg/mL using extract of rat brain cytosolic fraction (S1) | |
| UniProt accession no. | Q8IZP0  |
| Application: | Anti-ABI1 antibody produced in rabbit has been used in western blot analysis and immunofluorescence. |
| Application: | Rabbit anti- ABI1 antibody can be used for immunoblotting assays at a concentration of 0.5-1.0μg/ml using extracts from A431 and rat brain cells. |
| Biochem/physiol Actions: | Abelson interactor 1 (Abi1) is essential for the formation and activation of WAVE2 signaling complex. ABI1 and WAVE are essential for Rac-dependent membrane protrusion and macropinocytosis, whereas ABI1 and N-WASP regulate actin-based vesicular transport, EGF receptor endocytosis, and EGFR and transferrin receptor (TfR) cell surface distribution. ABI1 has been shown to act as dual regulator of WAVE and neural Wiskott-Aldrich syndrome protein (N-WASP) specific activities. ABI1 is an essential component of this signaling complex. It positively regulates WAVE activity and connects WAVE to Rac through assembly of the WAVE-ABI1-Nap1-PIR121 complex. Abi1 modulates actin reorganization and lamellipodia formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Abelson interactor 1 (Abi1) protein, a 65 kD-substrate of the eponymous Abelson non-receptor tyrosine kinase (c-Abl) is expressed mainly in the central nervous system. This gene is located on human chromosome 10p12. |
| General description: | Abi 1 is an adaptor protein that interacts with Wave2 to regulate actin polymerization. |
| Immunogen: | synthetic peptide corresponding to amino acids 163-180 of human ABI1, conjugated to KLH. The corresponding sequence is identical in several species including mouse, rat, and dog. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |