Adenosine Deaminase from bovine spleen
SIGMA/A5168 - Type IX, ammonium sulfate suspension, 150-200 units/mg protein
Synonym: Adenosine aminohydrolase
CAS Number: 9026-93-1
EC Number: 232-817-5
MDL Number: MFCD00081287
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | bovine spleen |
| foreign activity | 5′-AMP deaminase ≤0.01% |
| alkaline phosphatase ≤0.01% | |
| guanase ≤0.01% | |
| nucleoside phosphorylase ≤0.1% | |
| form | ammonium sulfate suspension |
| mol wt | 32.5-33 kDa |
| Quality Level | 200  |
| specific activity | 150-200 units/mg protein |
| storage temp. | 2-8°C |
| type | Type IX |
| UniProt accession no. | P56658  |
| Analysis Note: | Protein determined by biuret. |
| Application: | Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay. |
| Application: | Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease. |
| Biochem/physiol Actions: | Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease. |
| Biochem/physiol Actions: | Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis a |
| General description: | Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes |
| General description: | Molecular weight: 32.5-33 kDa pI: 4.85 |
| Other Notes: | One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C. |
| Physical form: | Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 150-200 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.4.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |