Anti-Human Lambda Light Chains (Bound and Free)−Peroxidase antibody produced in goat
SIGMA/A5175 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Human Lambda Light Chains (Bound and Free)-HRP
MDL Number: MFCD00162530
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human tonsil tissue sections were stained with 1:50 Anti-Human Lambda Light Chains (Bound and Free)-Peroxidase antibody produced in Goat (Cat. No. A5175).
ELISA Anti-Human Lambda Light Chains (Bound and Free)-Peroxidase antibody produced in Goat (Cat. No. A5175), was serially diluted to detect Human Lambda Light Chains by direct ELISA. Reactivity is observed with Human IgG, Human IgA, Human IgM and Human Lambda but not with Human Kappa, Mouse IgG and Rat IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:35,000 |
| dot blot: 1:40,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 |
| Application: | Anti-Human Lambda Light Chains (Bound and Free)?Peroxidase antibody produced in goat has been used in: • enzyme linked immunosorbent assay (ELISA) • immunohistology • dot blot • detecting and quantitating human lambda light chains (bound and free) via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) Western Blotting (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains. Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3’,5,5’-tetramethylbenz |
| Legal Information: | Ampliflu is a trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |