Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal
SIGMA/A5228 - clone 1A4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Actin, α-Smooth Muscle; Acta2 Antibody; Alpha Smooth Muscle Actin Antibody Sigma; SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal; Sma Antibody; alpha SMA; SMA
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Obesity and diabetes cause cognitive dysfunction in the absence of accelerated β:-amyloid deposition in a novel murine model of mixed or vascular dementia. By: Niedowicz, D. M., Reeves, V. L., et al. in Acta Neuropathol Commun, 2014. PubMed ID: 24916066 Image collected and cropped by CiteAb from the following publication, (http://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-64), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Obesity and diabetes cause cognitive dysfunction in the absence of accelerated β:-amyloid deposition in a novel murine model of mixed or vascular dementia. By: Niedowicz, D. M., Reeves, V. L., et al. in Acta Neuropathol Commun, 2014. PubMed ID: 24916066 Image collected and cropped by CiteAb from the following publication, (http://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-64), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: The polyphenols (-)-epigallocatechin-3-gallate and luteolin synergistically inhibit TGF-β:-induced myofibroblast phenotypes through RhoA and ERK inhibition. By: Gray, A. L., Stephens, C. A., et al. in PLoS One, 2014. PubMed ID: 25272043 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0109208), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 1:500 Monoclonal Anti-Actin, α-Smooth Muscle Clone: 1A4 (Cat. No. A5228) followed by Goat Anti-Mouse IgG (Fab)-FITC (Cat. No. F5262).
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Monoclonal Anti-Actin, α-Smooth Muscle Clone: 1A4 (Cat. No. A5228). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-Actin, α-Smooth Muscle Clone: 1A4 (Cat. No. A5228). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HS-68 2. RAT2 3. LA4 4. C2C12
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 1A4, monoclonal |
| conjugate | unconjugated |
| form | PBS solution |
| isotype | IgG2a |
| mol wt | antigen ~42 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, chicken, frog, canine, guinea pig, rabbit, bovine, goat, sheep, snake |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using smooth muscle cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections | |
| microarray: suitable | |
| western blot: suitable using smooth muscle cells | |
| UniProt accession no. | P62736  |
| Application: | Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal has been used in: • immunofluorescence staining • immunohistochemistry • immunoblotting • immunocytochemistry • enzyme linked immunosorbent assay (ELISA) • western blotting |
| Application: | Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees. |
| Application: | Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, cytokinesis, secretion, cytoplasmic streaming and phagocytosis. It has been shown that the relative proportion of actin isoforms are different in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle). |
| General description: | Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the NH2 terminal synthetic decapeptide of a smooth muscle actin, coupled to keyhole limpet hemocyanin (KLH). |
| General description: | The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. |
| Immunogen: | N-terminal synthetic decapeptide of α-smooth muscle actin. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |