Anti-ASF1 antibody, Mouse monoclonal
SIGMA/A5236 - clone 4A1/3, purified from hybridoma cell culture
Synonym: Anti-ASF1A; Anti-Silencing function 1 homolog A
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-ASF1 Clone: 4A1/3 (Cat. No. A5236). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Jurkat; 2. Raji; 3. NS1-mouse myeloma
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A1/3, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~23 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using whole extract of human Jurkat or Raji cells |
| UniProt accession no. | Q9Y294  |
| Application: | Monoclonal Anti-ASF1 antibody produced in mouse has been used in immunoblotting. |
| Biochem/physiol Actions: | ASF1 (anti-silencing function 1) protein is a key component of a histone donor complex. It interacts with histones H3 and H4 and functions together with a chromatin assembly factor 1 (CAF-1) during DNA replication and repair. ASF1 also contributes to genome stability during S phase. Cells lacking ASF1 were found to be sensitive to a number of genotoxic agents that predominantly cause DNA damage by interfering with DNA replication fork progression such as hydroxyurea and camptothecin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ASF1 is a histone chaperone protein that binds H3 and H4. It functions in nucleosome assembly. |
| General description: | Monoclonal Anti-ASF1 (mouse IgG2a isotype) is derived from the hybridoma 4A1/3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an ASF1A fusion protein. ASF1 (anti-silencing function 1) encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. |
| Immunogen: | ASF1A fusion protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |