Alcian Blue 8GX
SIGMA/A5268 - powder
Synonym: Alcian Blue; Ingrain Blue 1
CAS Number: 33864-99-2
Empirical Formula (Hill Notation): C56H68Cl4CuN16S4
Molecular Weight: 1298.86
EC Number: 251-705-7
MDL Number: MFCD00010720
Linear Formula: C56H68Cl4CuN16S4
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A5268-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A5268-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A5268-25G
| εmax | 425 at 615-630 nm in water |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | blue |
| composition | Dye content, 45-65% |
| form | powder |
| InChI | 1S/C56H68N16S4.4ClH.Cu/c1 |
| InChI key | CKLBXIYTBHXJEH-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | [Cl-].[Cl-].[Cl-].[Cl-].C |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | • Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices. • It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models. • It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species. |
| Biochem/physiol Actions: | The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1. |
| Features and Benefits: | Desired staining patterns can be obtained by manipulating pH and salt concentrations. |
| General description: | Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides. |
| Packaging: | 10, 25, 100 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 74240 |
| UNSPSC | 12171500 |