Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A5295 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Bovine IgG (whole molecule)−HRP
MDL Number: MFCD00162171
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of Rabbit spleen stained with Anti-Rabbit antibody produced in bovine followed by 1:1,000 Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5295).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:20,000 |
| dot blot: 1:80,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 |
| Application: | Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in: • enzyme-linked immunosorbent assay (ELISA) • bovine viral diarrhea virus (BVDV) plaque • focus-forming assays |
| Biochem/physiol Actions: | Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively. |
| Immunogen: | purified bovine IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |