Monoclonal Anti-β-Actin antibody produced in mouse
SIGMA/A5316 - clone AC-74, ascites fluid
Synonym: Actin Antibody Sigma; Beta Actin Antibody; Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse; Beta Actin Antibody Sigma; Sigma Beta Actin Antibody
MDL Number: MFCD00164531
Product Type: Chemical
Western Blotting JOURNAL CITATION: Balancing selection maintains a form of ERAP2 that undergoes nonsense-mediated decay and affects antigen presentation. By: Andrés, A. M., Dennis, M. Y., et al. in PLoS Genet, 2010. PubMed ID: 20976248 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1001157), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Epigenetic regulation of a murine retrotransposon by a dual histone modification mark. By: Brunmeir, R., Lagger, S., et al. in PLoS Genet, 2010. PubMed ID: 20442873 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1000927), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Pten dose dictates cancer progression in the prostate. By: Trotman, L. C., Niki, M., et al. in PLoS Biol, 2003. PubMed ID: 14691534 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pbio.0000059), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A5316). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 1:2,000 Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A5316) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:2,000 Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A5316). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence CFB cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:1,000 Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A5316). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | AC-74, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:1,000 using cultured human or chicken fibroblasts | |
| western blot: 1:5,000 using cultured human or chicken fibroblast cell extracts | |
| UniProt accession no. | P60709  |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. Mouse Monoclonal Anti-β-Actin antibody has been used for western blot analyses. The antibody can also be used for immunohistochemistry, indirect immunofluorescence (1:1,000) using cultured human or chicken fibroblasts, and indirect ELISA. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Monoclonal Anti-β-Actin antibody has been used in western blotting. |
| Application: | Mouse Monoclonal Anti-β-Actin antibody has been used for western blot analyses. The antibody can also be used for immunohistochemistry, indirect immunofluorescence (1:1,000) using cultured human or chicken fibroblasts, and indirect ELISA. |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder. It recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1. Monoclonal Anti-β-Actin (mouse IgG2a isotype) is derived from the AC-74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting. |
| Immunogen: | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | The product is provided as ascites fluid containing 0.1% sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |