Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
SIGMA/A5420 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti Goat Igg; Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
MDL Number: MFCD00162340
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human tonsil sections stained with Anti-Human IgG (whole molecule) antibody produced in goat (Cat. No. I1011) followed by HRP-Anti Goat IgG (H+L) (Rb) antibody (Cat. No. A5420).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| dot blot: 1:80,000-1:160,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| Application: | LNCAP cell lysates were analyzed by western blot using HRP conjugated rabbit anti-goat IgG as the secondary at a 1:7500 dilution in TBSt for 1 hour at room temperature. |
| Application: | Rabbit Anti-Goat IgG (whole molecule)-Peroxidase antibody has been used for immunoblotting assays. The antibody can also be used for direct ELISA (1:40,000), dot blot (1:80,000-1:160,000) and immunohistochemistry (1:200) assays. Anti-Goat IgG (whole molecule)–Peroxidase antibody has been used in • western blotting • immunohistochemical and immunofluorescent analysis • visualization of cross-reactivity • protein analysis • indirect ELISA for detection of specific IgG against C. pseudotuberculosis • immunoprecipitation (IP) |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) glycoprotein antibodies modulates several immune responses. It mainly participates in hypersensitivity type II and type III. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein and a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody binds to all goat Igs. |
| Immunogen: | Purified goat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Specificity: | Specificity of Anti-Goat IgG- Peroxidase is determined by immunoelectrophoresis (IEP) versus normal goat serum and goat IgG. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS07,GHS07 |
| Signal word | Warning-Warning |
| Hazard statements | H317 - H317 |
| Precautionary statements | P261 - P272 - P280 - P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |