Monoclonal Anti-β-Actin antibody produced in mouse
SIGMA/A5441 - clone AC-15, ascites fluid
Synonym: Monoclonal Anti-β-Actin; Anti beta actin monoclonal antibody
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions. By: Nakamura, F. in BMC Cell Biol, 2001. PubMed ID: 11425343 Image collected and cropped by CiteAb from this publication, provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β:1. By: Starsíchová, A., Kubala, L., et al. in Biol Proced Online, 2009. PubMed ID: 19756912 Image collected and cropped by CiteAb from the following publication, (http://www.biologicalproceduresonline.com/content/11/1/316), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions. By: Nakamura, F. in BMC Cell Biol, 2001. PubMed ID: 11425343 Image collected and cropped by CiteAb from this publication, provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
a-c. Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-β-Actin, Clone No. AC-15 (Cat. No. A5441) (green) and polyclonal antibodies to chicken gizzard myosin (red).
d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-α-Actinin (green, d, e; red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-β-Actin, Clone No. AC-15 (Cat. No. A5441) (f, green). Bars, 10 mm.
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.
d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-α-Actinin (green, d, e; red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-β-Actin, Clone No. AC-15 (Cat. No. A5441) (f, green). Bars, 10 mm.
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.
Immunofluorescence
Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-β Actin, Clone No. AC-15 (Cat. No. A5441).
Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-β Actin, Clone No. AC-15 (Cat. No. A5441).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 1:1,000 Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A5441) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence HS-68 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:1,000 Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A5441). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A5441). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | AC-15, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 42 kDa |
| Quality Level | 300  |
| shipped in | dry ice |
| should not react with | Dictyostelium discoideum |
| species reactivity | sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts | |
| western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts | |
| UniProt accession no. | P60709  |
| Application: | Monoclonal Anti-β-Actin antibody produced in mouse has also been used: • in immunofluorescence staining • in immunoblotting • in immunohistochemistry • as a control for protein arrays |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. |
| Application: | Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, cytokinesis, cytoplasmic streaming, secretion and phagocytosis. The actin in cells of various species and tissue origin is very similar in its immunological and physical properties. |
| Biochem/physiol Actions: | Monoclonal Anti β-Actin antibody recognizes an epitope located on the N-terminal end of the β-isoform of actin. The antibody specifically labels β-actin in a wide variety of tissues and species using immunoblotting (42 kDa), immunofluorescent staining of cultured cell lines, and immunohistochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| General description: | Monoclonal Anti-β-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac, and α-smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non-muscle). Actin isoforms show >90% overall sequence homology, but only 50−60% homology in their 18 NH2-terminal residues. The NH2-terminal region of actin appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding. |
| Immunogen: | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Supplied as ascites fluid with 15mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |