Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A5545 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Guinea pig IgG (whole molecule)−HRP
MDL Number: MFCD00162368
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human pancreus tissue sections were stained with 1:100 Anti-Insulin antibody produced in Guinea Pig (Cat. No. I8510) followed by 1:400 Anti-Guinea Pig IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5545).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000-1:80,000 |
| dot blot: 1:50,000-1:100,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800 |
| Application: | Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting. |
| Biochem/physiol Actions: | IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) is the major class and most abundant immunoglobulin of immune system released by plasma cells. IgG mediate immune response with respect to the type of antigen and is found in blood or extracellular fluid. IgG defends against bacterial and viral infections. Maternal IgG crosses from mother to fetus through the placental barrier by pinocytosis to provide passive immunity for the neonate and protect against infections. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism. It mainly helps in immune defense. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions. |
| Biochem/physiol Actions: | Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. It has two subclasses of IgG- IgG1 and IgG2. |
| Immunogen: | guinea pig IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Preparation Note: | Store at -20 °C for long term use. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |