Anti-Water Channel Aquaporin 1 antibody produced in rabbit
SIGMA/A5560 - IgG fraction of antiserum, lyophilized powder
MDL Number: MFCD02262541
Product Type: Chemical
Immunohistochemistry Anti-Aquaporin 1: Cat. No. A5560: Immunohistochemical staining of paraffin-embedded Rat kidney sections using Anti-Aquaporin 1 antibody, (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.
Immunoblotting Anti-Aquaporin 1: Cat. No. A5560: Western blot analysis of Rat kidney membranes. Lanes 1. Anti-Aquaporin 1 antibody, (1:400). 2. Anti-Aquaporin 1 antibody, preincubated with the control peptide antigen.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| western blot (chemiluminescent): 1:400-1:800 | |
| UniProt accession no. | P29972  |
| Application: | Rabbit Anti-Water Channel Aquaporin 1 antibody has been used for immunocytochemical assays. The product can also be used for immunohistochemistry and western blot. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Aquaporins are transmembrane proteins composed of 6 α helices that regulate the flow of water across the membrane. AQP1 is highly expressed on the apical membrane of kidneys. |
| General description: | Rabbit Anti-Water Channel Aquaporin 1 antibody binds to human and rat aquaporin 1. |
| Immunogen: | synthetic peptide corresponding to amino acids 243-261 of human aquaporin 1 conjugated to KLH with glutaraldehyde. The epitope is identical in human, rodent, and cattle and only slightly different in frog. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, and 0.025% sodium azide |
| Preparation Note: | Isolated on immobilized Protein A |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |