Monoclonal Anti-α1-Acid Glycoprotein antibody produced in mouse
SIGMA/A5566 - clone AGP-47, ascites fluid
MDL Number: MFCD00162118
Product Type: Chemical
Western Blotting Human plasma extract were separated on SDS-PAGE and probed with Anti-α1-Acid Glycoprotein antibody, Mouse monoclonal, Clone: AGP-47 (Cat. No. A5566). The antibody was developed using 1:20,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:10,000 antibody 2. 1:5,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AGP-47, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | canine, bovine |
| species reactivity | human, baboon (weakly) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| western blot: 1:10,000 | |
| UniProt accession no. | P02763  |
| Biochem/physiol Actions: | α1-Acid Glycoprotein (AGP) associates with haptoglobin and C-reactive protein, which indicates inflammation. Higher levels of AGP is associated with various inflammatory diseases, trauma, malignancies, myocardial infarction, rheumatoid arthritis, after major surgery, and in cases of chronic pain. This protein is capable of binding and transporting basic and neutral drugs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-α1-Acid Glycoprotein (mouse IgG1 isotype) is derived from the AGP-47 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. α1-Acid Glycoprotein (AGP) is an acute-phase plasma protein. This protein is mainly produced in the hepatocytes. AGP1 gene is mapped on the human chromosome at 9q32. |
| Immunogen: | human α1-acid glycoprotein (AGP). |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| Specificity: | Recognizes an epitope located on the 44 kDa subunit of denatured-reduced AGP using the immunoblotting technique. It does not cross react with human serum amyloid P component, human haptoglobin, human C-reactive protein, and human IgG. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |