Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A5588 - clone HIS-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-polyHistidine; Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse; Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Product Type: Chemical
Western Blotting Whole extract of 293T cells were separated on SDS-PAGE, probed and developed with Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody produced in Mouse, Clone: HIS-1 (Cat. No. A5588). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HIS-1, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
| Application: | Antibody is suitable for ELISA, immunoblotting and dot blotting assays. |
| Biochem/physiol Actions: | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
| General description: | The Monoclonal Anti-polyHistidine-Alkali |
| Immunogen: | recombinant polyHistidine tagged fusion protein. |
| Legal Information: | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |