Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A5588 - clone HIS-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-polyHistidine; Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse; Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Product Type: Chemical
antibody form | purified from hybridoma cell culture |
antibody product type | primary antibodies |
biological source | mouse |
clone | HIS-1, monoclonal |
conjugate | alkaline phosphatase conjugate |
form | buffered aqueous glycerol solution |
isotype | IgG2a |
Quality Level | 200 |
shipped in | wet ice |
storage temp. | 2-8°C |
technique(s) | western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
Application: | Antibody is suitable for ELISA, immunoblotting and dot blotting assays. |
General description: | The Monoclonal Anti-polyHistidine-Alkali |
Immunogen: | recombinant polyHistidine tagged fusion protein. |
Legal Information: | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
Specificity: | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |