Anti-c-Myc−Peroxidase antibody produced in rabbit
SIGMA/A5598 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-c-Myc antibody produced in rabbit
MDL Number: MFCD00239651
Product Type: Chemical
Western Blotting Whole extract of E. coli cells expressing c-Myc tag were separated on SDS-PAGE. The antibody was probed and developed with Anti-c-Myc-Peroxidase antibody produced in Rabbit (Cat. No. A5598). Lanes 1. 1:5,000 antibody 2. 1:7,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.8-4 mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:5,000 |
| UniProt accession no. | P01106  |
| Application: | Antibody is suitable for immunoblotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser- |
| General description: | The Anti-c-Myc peroxidase conjugate rabbit antibody reacts with c-Myc tagged fusion proteins. It recognizes the c-Myc tag sequence expressed at either the amino- or the carboxyl-terminus. |
| Immunogen: | peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M sodium phosphate buffer saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |