Anti-Arp1α/Centractin antibody produced in rabbit
SIGMA/A5601 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD04040182
Product Type: Chemical
Immunoblotting
Whole cell extract of A431 cells was separated on SDS-PAGE and blotted with Rabbit Anti-Arp1/Centractin (Cat. No. A5601). The antibody was developed with Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lane A) Antibody dilution 1:1,000; (Lane B) Antibody dilution 1:1,000 + 5 mg/mL, Arp1/Centractin immunizing peptide
Whole cell extract of A431 cells was separated on SDS-PAGE and blotted with Rabbit Anti-Arp1/Centractin (Cat. No. A5601). The antibody was developed with Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lane A) Antibody dilution 1:1,000; (Lane B) Antibody dilution 1:1,000 + 5 mg/mL, Arp1/Centractin immunizing peptide
Immunohistochemistry Anti-Arp1α/Centractin: Cat. No. A5601: Immunoperoxidase staining of Anti-Arp1α/Centractin in formalin fixed, paraffin-embedded rat kidney at 1.8 μg/mL.
Immunohistochemistry Anti-Arp1α/Centractin: Cat. No. A5601: Immunoperoxidase staining of Anti-Arp1α/Centractin in formalin fixed, paraffin-embedded rat duodenum at 1.8 μg/mL.
Immunofluorescence NIH-3T3 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Arp1α/Centractin antibody produced in Rabbit (Cat. No. A5601) followed by 1:500 Anti-Rabbit IgG-Cy3 antibody.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:100 using mouse fibroblasts NIH3T3 cell line |
| microarray: suitable | |
| western blot: 1:1,000 using rat brain cytosol extract or whole cell extract of human epidermoid carcinoma A431 cell line. | |
| UniProt accession no. | P61163  |
| Application: | Anti-Arp1α/Centractin antibody produced in rabbit has been used in immunoblotting and immunofluorescence staining. |
| Biochem/physiol Actions: | Arp1α or Centractin is an actin-related, ubiquitious protein that forms a part of the dynactin complex and is associated with centrosomes. Studies have reported that centractin interacts with spectrin and subsequently provides a link for dynactin to associate with organelles in cells. |
| Biochem/physiol Actions: | The actin-related proteins (Arp)1α/dynactin complex regulates dyne mediated vesicle movement on microtubules as well as spindle assembly and cell division. Arp1α overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of Arp1α/dynactin complex in progression through the prometaphase of mitosis. Expression of mutant Arp1a protein has no effect on mitotic cells, but results in microtubule disruption in interphase cells, by destabilizing the interaction between Arp1a and components of the dynactin complex and nuclear mitotic apparatus protein (NuMA). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The actin-related protein Arp1α (centractin, actin-RPV), a member of the superfamily of actin-related proteins (Arp), is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. It has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. |
| Immunogen: | synthetic peptide corresponding to the mid-region of human Arp1α/centractin (amino acids 222-240). This sequence is identical in mouse and dog Arp1α/centractin, highly conserved (89% identity) in the α-centractin isoform, and not found in known actin isoforms. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |