Monoclonal Anti-polyHistidine–Agarose antibody produced in mouse
SIGMA/A5713 - clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins
Synonym: Monoclonal Anti-polyHistidine; Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Product Type: Chemical
Immunoprecipitation Monoclonal Anti-polyHistidine-Agarose antibody produced in Mouse, Clone: HIS-1 (Cat. No. A5713), was used to immunoprecipitate polyHistidine tagged fusion protein from Bacterial culture extract. Detection antibody: 1:2,000 Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody produced in Mouse, Clone: HIS-1 (Cat. No. A5588). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 4 μg/mL antibody 4. 8 μg/mL antibody 5. 10 μg/mLl antibody 6. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| capacity | 15-30 nmol/mL binding capacity |
| clone | HIS-1, monoclonal |
| conjugate | agarose conjugate |
| form | PBS suspension |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| suitability | suitable for purification of HIS tagged recombinant proteins |
| target post-translational modification | unmodified |
| Application: | Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| General description: | This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins. |
| Immunogen: | recombinant polyHistidine tagged fusion protein. |
| Legal Information: | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
| Physical form: | Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume. |
| Specificity: | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |