Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A5795 - affinity isolated antibody, buffered aqueous solution
Synonym: HRP Rabbit Anti-Rat IgG (whole molecule)
MDL Number: MFCD00162813
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with anti-human IgG antibody produced in Rat followed by 1:750 Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5795).
Western Blotting Whole extract of 293T cells transfected with Flag were separated on SDS-PAGE and probed with 1 μg/mL Anti-FLAG® antibody, Rat monoclonal, Clone: 6F7 (Cat. No. SAB4200071). The antibody was developed using Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5795). Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750 | |
| western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract |
| Application: | Anti-Rat IgG (whole molecule)-Peroxidase antibody has been used as a secondary antibody in immunohistochemical applications at a dilution of 1:1500. The product has also been used for western blot analysis at 1:20,000. Furthermore, the antibody can be used for direct ELISA at 1:50,000. |
| Application: | Anti-Rat IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis. |
| Application: | HA-tagged proteins over expressed in S. cerevisiae were detected by western blot using HRP-conjugated rabbit anti-rat IgG as the secondary antibody. |
| Biochem/physiol Actions: | Immunoglobulin (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG participates in hypersensitivity type II and type III. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG. |
| Immunogen: | purified rat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT |
| Preparation Note: | Adsorbed with human IgG to reduce background staining with human samples. |
| Preparation Note: | Prepared by two-step glutaraldehyde method. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |