Monoclonal Anti-c-Abl antibody produced in mouse
SIGMA/A5844 - clone ABL-148, ascites fluid
Synonym: Anti-ABL; Anti-BCR-ABL; Anti-CHDSKM; Anti-JTK7; Anti-bcr/abl; Anti-c-ABL; Anti-c-ABL1; Anti-p150; Anti-v-abl
MDL Number: MFCD00239646
Product Type: Chemical
Western Blotting Whole extract of G361 Human melanoma cells were separated on SDS-PAGE and probed with Monoclonal Anti-c-Abl antibody produced in Mouse, Clone: ABL-148 (Cat. No. A5844). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:4,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ABL-148, monoclonal |
| conjugate | unconjugated |
| isotype | IgG2a |
| mol wt | antigen 145 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, rat, human, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:2,000 using human melanoma cell extract | |
| UniProt accession no. | P00519  |
| Application: | Monoclonal anti-c-Abl antibody can be used in western blotting (diluted 1:2000) using melanoma cell extract from human. It can also be used in microarray and flow cytometry. Monoclonal anti-c-Abl antibody can be used for studying the mechanism of signaling pathways involving c-Abl. It may also be used for immunoprecipitation and immunocytochemistry. |
| Biochem/physiol Actions: | Cytoplasmic c-Abl regulates SH2/SH3 adaptor protein cytoskeleton-associated adaptor protein (Crk) and the Crk-binding protein p130cas. Nuclear c-Abl has been implicated in the regulation of cell cycle-dependent and DNA damage-induced gene expression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | c-Abl is a proto-oncogene product that belongs to Tyr protein kinase family. It has a crucial role in different cellular processes like- differentiation, adhesion and regulates DNA damage-induced apoptosis. |
| General description: | The c-Abl protein contains three high mobility group-like domains that bind to AT-rich DNA in a cooperative manner. |
| Immunogen: | recombinant c-Abl, SH2 domain. |
| Specificity: | Mouse anti-c-Abl antibody reacts specifically with an epitope present in the SH2 domain of the c-Abl. The product has also shown reactivity for c-Abl of monkey, rat, bovine, mouse and human. |
| Target description: | c-Abl is a non-receptor tyrosine kinase with both cytoplasmic and nuclear functions. The Abl oncogen has been implicated in several human leukemias including nearly all chronic myelocytic leukemias. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |