Anti-AFX (FOXO4) antibody, Mouse monoclonal
SIGMA/A5854 - clone AF3.10, purified from hybridoma cell culture
MDL Number: MFCD03791410
Product Type: Chemical
Western Blotting Whole extract of COS-7 cells expressing Human AFX were separated on SDS-PAGE and probed with Anti-AFX (FOXO4) antibody, Mouse monoclonal, Clone: AF3.10 (Cat. No. A5854). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 2 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AF3.10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using COS-7 cell extracts expressing human AFX | |
| UniProt accession no. | P98177  |
| Application: | Monoclonal Anti-AFX (FOXO4) antibody can be used for western blot assays at 0.5-1μg/ml. The antibody is also suitable for use in indirect ELISA, immunocytochemistry and microarray applications. |
| Biochem/physiol Actions: | Anti-AFX (FOXO4) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma AF3.10 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. AFX plays a central role in the regulation of cell proliferation. It integrates signals from PI3K/PKB and Ras/Ral signaling pathways to regulate transcription of the cell cycle inhibitor p27kip1. AFX activation results in increased expression of p27kip1, resulting in cell cycle arrest. Overexpression of AFX blocks cells cycle progression at G1 by a p27kip1 dependent mechanism. |
| Biochem/physiol Actions: | Monoclonal Anti-AFX (FOXO4) recognizes human AFX. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Forkhead box O4 (FOXO4) belongs to the FOXO transcription factor family that regulates growth, cell cycle, metabolism, stress tolerance, aging and apoptosis in cells. Studies have reported that USP7 modulates FOXO4 transcription. Moreover, it has been demonstrated that peroxide stress acetylates FOXO4, whereas the longevity protein hSir2SIRT1 deacetylates it. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |