Antibiotic Antimycotic Solution (100×), Stabilized
SIGMA/A5955 - with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered, BioReagent, suitable for cell culture
Synonym: Penicillin-
MDL Number: MFCD00130520
Product Type: Chemical
| antibiotic activity spectrum | fungi |
| Gram-negative bacteria | |
| Gram-positive bacteria | |
| yeast | |
| application(s) | agriculture |
| color | yellow |
| form | suspension |
| impurities | endotoxin, tested |
| mode of action | cell wall synthesis | interferes |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | dry ice |
| sterility | 0.1 μm filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Antibiotic antimycotic solution has been used:• as a supplement in different types of cell culture media. • as a component of phosphate buffer saline (PBS) sterile solution to prevent contamination of periodontal ligament fibroblasts (PDLF) tissues that are isolated and cultured in the experiments. • as a component of storage solution for preserving tracheal segments harvested for subsequent experimental investigation. |
| Biochem/physiol Actions: | Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out. Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts. |
| General description: | Antibiotic Antimycotic Solution is formulated to contain 10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and is solubilized in a proprietary citrate buffer. It is recommended for use in cell culture applications at 10mL/L. Antibioticantimycotic solution is generally used to prevent the growth of harmful microorganisms including bacteria and fungi in cell culture media. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |