Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A5963 - clone 9E10, purified from hybridoma cell culture
Synonym: Anti-c-Myc; Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse
Product Type: Chemical
Western Blotting Whole extract of E.Coli TG-1 cells c-Myc tagged fusion protein were separated on SDS-PAGE and probed and developed with Monoclonal Anti-c-Myc antibody produced in Mouse, Clone: 9E10 (Cat. No. A5963). Lanes 1. 1:200 antibody 2. 1:100 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9E10, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 1:100 using a myc-tagged fusion protein. | |
| UniProt accession no. | P01106  |
| Application: | Anti-c-Myc-Alkaline Phosphatase antibody has been used in • enzyme linked immunosorbent assay (ELISA) • immunohistochemical labeling • immunostaining • western blotting |
| Application: | Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications. |
| Biochem/physiol Actions: | The c-myc oncogene product(p62c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei. |
| Immunogen: | synthetic peptide of the human p62c-Myc protein. |
| Physical form: | Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |