Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse
SIGMA/A5964 - clone PT-66, purified immunoglobulin, lyophilized powder
Synonym: Monoclonal Anti-Phosphotyrosine; Phospho-Tyr; Phospho-tyrosine; p-Tyr
Product Type: Chemical
ELISA Monoclonal Anti-Phosphotyrosine-Peroxidase antibody produced in Mouse, Clone: PT-66 (Cat. No. A5964) was serially diluted to detect Phosphotyrosine by direct ELISA. Reactivity is observed only with Phosphothyrosine-BSA but not with Phosphoserine-BSA, Phosphothreonine-BSA and BSA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PT-66, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| packaging | vial of 0.2 mL conjugate |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:60,000 using Phosphotyrosine-BSA |
| dot blot: 1:40,000-1:200,000 using phosphotyrosine-BSA using chromogenic and chemiluminescent substrates, respectively |
| Application: | Monoclonal Anti-Phosphotyrosine-Pero • enzyme linked immunosorbent assay (ELISA) • dot blot • Chemiluminescence dot blot • kinase assay |
| Biochem/physiol Actions: | Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine–pero |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. |
| General description: | Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the PT-66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphotyrosine-BSA conjugate. |
| Immunogen: | phosphotyrosine conjugated to BSA |
| Physical form: | Lyophilized from a solution containing 1% bovine serum albumin and 0.05% MIT in 0.01 M sodium phosphate buffered saline. |
| Specificity: | Mouse anti-phosphotyrosine-pero |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |