Anti-ARP3 antibody, Mouse monoclonal
SIGMA/A5979 - clone FMS338, purified from hybridoma cell culture
MDL Number: MFCD04118400
Product Type: Chemical
Immunofluorescence
C2C12 cells were incubated with (A) Monoclonal Anti-ARP2 (Cat. No. A6104) at 10 μg/mL or with (B) Monoclonal Anti-ARP3 (Cat. No. A5979) at 10 μg/mL) and developed with Goat Anti-Mouse IgG-Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
From Drs. Craig Stovold and Laura M. Machesky, School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, UK.
C2C12 cells were incubated with (A) Monoclonal Anti-ARP2 (Cat. No. A6104) at 10 μg/mL or with (B) Monoclonal Anti-ARP3 (Cat. No. A5979) at 10 μg/mL) and developed with Goat Anti-Mouse IgG-Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
From Drs. Craig Stovold and Laura M. Machesky, School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, UK.
Western Blotting Whole extract of Mouse myoblast C2 cells were separated on SDS-PAGE and probed with Monoclonal Anti-ARP3 antibody produced in Mouse, Clone: FMS338 (Cat. No. A5979). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FMS338, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 40-45 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using total cell extract of mouse myoblast C2 cells | |
| UniProt accession no. | P61158  |
| Application: | Monoclonal Anti-ARP3 antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunocytochemistry • immunoblotting • immunoprecipitation • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | The actin-related protein-2/3 (ARP2/3) is part of Wiskott-Aldrich Syndrome protein (WASP) signaling in mediating polymerization state of actin filaments. It has been found that ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar2 is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events. |
| Biochem/physiol Actions: | The ARP2/3 complex consists of seven distinct proteins (Arp2 and Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc) that modulate polymerization of cellular actin filaments. ARP3 regulates actin nucleation, and is involved in directing the movement of Listeria monocytogenes in infected host cells. Monoclonal Anti-ARP3 antibody binds to human, canine, rat, and mouse ARP3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ARP3 (mouse IgG2b isotype) is derived from the hybridoma FMS338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP3. Arp3 is mapped to human chromosome 2q14.1. |
| Immunogen: | recombinant human ARP3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |