Apotryptophanase from Escherichia coli
SIGMA/A6007 - soluble powder, 75-150 units/mg solid
Synonym: Tryptophanase from Escherichia coli; L-Tryptophan indole-lyase (deaminating)
CAS Number: 9024-00-4
MDL Number: MFCD00130539
Product Type: Chemical
Unit Definition One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate (PRP). Stoichiometry: L-Tryptophan + H2O → Indole + Pyruvate + Ammonia
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6007-25MG
| biological source | Escherichia coli |
| form | soluble powder |
| Quality Level | 200  |
| specific activity | 75-150 units/mg solid |
| storage temp. | −20°C |
| Application: | Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency . |
| Biochem/physiol Actions: | Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase . |
| Other Notes: | One unit releases one μg of indole from |
| Packaging: | 25 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 75-150 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.1.99.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |