Acridine Orange hemi(zinc chloride) salt

SIGMA/A6014 - For nucleic acid staining in cells or gels

Synonym: 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt; 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt); Basic Orange 14

CAS Number: 10127-02-3
Empirical Formula (Hill Notation): C₁₇H₁₉N₃ · HCl · 0.5ZnCl₂
Molecular Weight: 369.96
EC Number: 233-353-6
MDL Number: MFCD00081043
Linear Formula: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A6014-10G	10 g	$98.90 1/EA	Add To Favorites
45-A6014-25G	25 g	$229.00 1/EA	Add To Favorites
45-A6014-100G	100 g	$524.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6014-100G

Properties
Descriptions
Safety Info.
Basic Data

composition	Dye content, ~80%
form	powder
InChI	1S/2C17H19N3.4ClH.Zn/c21-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h25-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
InChI key	RAHGLSRJKRXOSY-UHFFFAOYSA-L
product line	BioReagent
Quality Level	200
SMILES string	Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
solubility	4 mg/mL (2-methoxyethanol (EGME))
	ethanol: 2 mg/mL
	water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
storage temp.	room temp
suitability	suitable for flow cytometry
	suitable for microscopy
technique(s)	nucleic acid detection: suitable

Application:	Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Application:	Suitable for • detection of nucleic acids separated by gel electrophoresis • fluorescence and epifluorescence microscopy • analysis of mitochondria and lysosomes by flow cytometry • DNA staining in apoptosis studies
Features and Benefits:	• 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels • differential staining of single- and double-stranded polynucleotides
General description:	Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Packaging:	10, 25, 100 g in glass bottle
Principle:	Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Symbol	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	Xn
Risk Statements	68
Safety Statements	36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
Colour Index Number	46005
UNSPSC	12352200

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