Anti-ATM antibody, Mouse monoclonal
SIGMA/A6093 - clone SYR6D4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-ATM antibody produced in mouse; Anti-
MDL Number: MFCD01321740
Product Type: Chemical
Western Blotting Whole extract of G361 cells were separated on SDS-PAGE and probed with Anti-ATM antibody, Mouse monoclonal (Cat. No. A6093) followed by 1:30,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SYR6D4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 350 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using a whole cell extract of an ATM high-producer human melanoma cell line | |
| UniProt accession no. | Q13315  |
| Application: | Anti-ATM antibody, Mouse monoclonal has been used in: • immunoblotting • immunocytochemistry • immunoprecipitation |
| Application: | Endogenous ATM was immunoprecipitated from whole cell lysates using monoclonal anti-ATM (SYR6D4). Immunoprecipitates were used for an ATM kinase assay. |
| Application: | Mouse Monoclonal Anti-ATM antibody can be used for western blot at 1-2μg/mL using a whole cell extracts of melanoma cell lines. The antibody can also be used for microarray, immunocytochemistry and immunoprecipitation assays. |
| Biochem/physiol Actions: | Ataxia-telangiectasia (A-T) cells exhibit hypersensitivity to ionizing radiation and multiple defects responding to DNA double-strand breaks. In addition, A-T cells exhibit a variety of cellular abnormalities in culture, including cytoskeletal defects, abnormalities in the plasma membrane and defects in intracellular signaling. ATM kinase activity is enhanced immediately after exposure of cells to DNA double strand break (DSB)-inducing agents and a fraction of it is localized to nuclear aggregates, colocalized with the phosphorylated form of histone H2AX and Nbs1 protein. It binds to β-adaptin, one of the components of the AP-2 adaptor complex, which is involved in clathrin-mediated endocytosis of receptors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ataxia-telangiectasia (A-T) is a rare human autosomal recessive disease with a pleiotropic phenotype characterized by cerebellar degeneration, oculocutaneous telangiectasias, immune dysfunction, genomic instability, cancer predisposition, radiation sensitivity, and premature aging. The gene mutated in A-T, ATM (A-T, mutated), encodes a 350-370 kDa protein. The C-terminal region of the protein has extensive homology to the catalytic domain of PI-3 kinase. ATM is predominantly nuclear and localizes to cytoplasmic vesicles. ATM expression is absent or expressed at very low levels in A-T cells. |
| General description: | Ataxia-telangiectasia, mutated (ATM) is a protein kinase that may regulate cell cycle and the response to DNA damage. ATM is known to associate with β-adaptin and β-NAP and hence, may modulate intracellular transport of proteins and vesicles. Mouse Monoclonal Anti-ATM antibody recognizes human ATM (approx. 350kDa). |
| Physical form: | Solution 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |