Anti-ARP2 antibody, Mouse monoclonal
SIGMA/A6104 - clone FMS96, purified from hybridoma cell culture
MDL Number: MFCD03097266
Product Type: Chemical
Immunofluoresence
ARP2 and ARP3 detection in C2C12 cells (mouse myoblasts) by immunofluoresence labeling. C2C12 cells were incubated with (A) Monoclonal Anti-ARP2 (Cat. No. A6104) 10 μg/mL or with (B) Monoclonal Anti-ARP3 (Cat. No. A5979) 10 μg/mL followed by Goat Anti-Mouse IgG, Alexa Fluor™ 488 (green staining). The red staining is obtained by staining actin with Alexa Fluor™ 546 phalloidin.
Pictures are courtesy of Drs. Craig Stovold and Laura M. Machesky, School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, UK.
ARP2 and ARP3 detection in C2C12 cells (mouse myoblasts) by immunofluoresence labeling. C2C12 cells were incubated with (A) Monoclonal Anti-ARP2 (Cat. No. A6104) 10 μg/mL or with (B) Monoclonal Anti-ARP3 (Cat. No. A5979) 10 μg/mL followed by Goat Anti-Mouse IgG, Alexa Fluor™ 488 (green staining). The red staining is obtained by staining actin with Alexa Fluor™ 546 phalloidin.
Pictures are courtesy of Drs. Craig Stovold and Laura M. Machesky, School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, UK.
Western Blotting Whole extract of C2 Mouse myoblast cells were separated on SDS-PAGE and probed with Anti-ARP2 antibody, Mouse monoclonal, Clone: FMS96 (Cat. No. A6104). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 2 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FMS96, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 40-45 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total cell extract of C2 mouse myoblast cells | |
| UniProt accession no. | P61160  |
| Application: | Anti-ARP2 antibody, Mouse monoclonal has been used in: • Immunoblotting • Immunocytochemistry • Enzyme linked immuosorbent assay |
| Biochem/physiol Actions: | Actin-related protein 2 (ARP2) is a part of ARP2/3 complex and play a key role in actin filament formation. It mediates changes in the polymerization state of actin filaments. The Wiskott-Aldrich Syndrome protein (WASP) regulates ARP2/3 complex of proteins. The ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-ARP2 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FMS96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP2. |
| General description: | ARP2 is part of a complex with 7 subunits called ARP2/3. ARP2 is an actin-related protein that function to regulate the actin cytoskeleton. |
| General description: | Mouse Monoclonal Anti-ARP2 antibody binds to human, canine, rat, and mouse ARP2, 40-45kDa. |
| Immunogen: | recombinant human ARP2 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |