Apyrase from potatoes
SIGMA/A6132 - ATPase ≥3.0 units/mg protein, lyophilized powder
Synonym: Adenosine 5′-diphosphatase; Adenosine 5′-triphosphatase
CAS Number: 9000-95-7
EC Number: 232-569-8
MDL Number: MFCD00130542
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6132-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6132-2KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6132-500UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6132-5KU
| ATPase activity | ≥3.0 units/mg protein |
| biological source | potato |
| composition | Protein, 30-60% |
| form | lyophilized powder |
| Quality Level | 300  |
| storage temp. | −20°C |
| type | Grade I |
| Application: | Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . |
| Application: | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. |
| Biochem/physiol Actions: | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. |
| Other Notes: | Mixture of high and low ratio isoenzymes. |
| Other Notes: | One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C |
| Packaging: | 200 units in glass bottle |
| Packaging: | 500, 1000, 2000, 5000 units in poly bottle |
| Packaging: | Sold on the basis of ATPase units. |
| Physical form: | Partially purified, lyophilized powder containing potassium succinate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | ATPase activity: ≥3.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |