Antipain dihydrochloride from microbial source
SIGMA/A6191 - protease inhibitor
Synonym: N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe
CAS Number: 37682-72-7
Empirical Formula (Hill Notation): C27H44N10O6 · 2 HCl
Molecular Weight: 677.62
MDL Number: MFCD00135957
Linear Formula: C27H44N10O6 · 2 HCl
Product Type: Chemical
biological source | Streptomyces sp. |
form | lyophilized powder |
InChI | 1S/C27H44N10O6.2ClH/c1-16 |
InChI key | YAHXZYICKJUJEO-BXLPLHKWSA |
mode of action | enzyme | inhibits |
potency | 1.4 μM Ki |
Quality Level | 300 |
SMILES string | Cl[H].Cl[H].[H]C(=O)C(CCC |
solubility | 1-butanol: soluble |
1-propanol: soluble | |
DMSO: soluble | |
ethanol: soluble | |
H2O: 50 mg/mL | |
methanol: soluble | |
storage temp. | −20°C |
Application: | Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis. |
Application: | Concentrations for 50% inhibition (μg/ml): papain, 0.16 trypsin, 0.26 cathepsin A, 1.19 cathepsin B, 0.59 cathepsin D, 125 plasmin, >93 chymotrypsin and pepsin, >250 It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM |
Biochem/physiol Actions: | Antipain dihydrochloride also inhibits the action of papain and cathespsin B. |
Biochem/physiol Actions: | Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells. |
General description: | Chemical structure: peptide |
Packaging: | 1, 5, 25, 100 mg in glass bottle |
Preparation Note: | Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.8 A stock solution in water or buffer is stable for about a month at -20 °C. Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization. |
Preparation Note: | Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352202 |