Antipain dihydrochloride from microbial source

SIGMA/A6191 - protease inhibitor

Synonym: N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe

CAS Number: 37682-72-7
Empirical Formula (Hill Notation): C₂₇H₄₄N₁₀O₆ · 2 HCl
Molecular Weight: 677.62
MDL Number: MFCD00135957
Linear Formula: C₂₇H₄₄N₁₀O₆ · 2 HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A6191-1MG	1 mg	$70.90 1/EA	Add To Favorites
45-A6191-5MG	5 mg	$105.00 1/EA	Add To Favorites
45-A6191-25MG	25 mg	$394.00 1/EA	Add To Favorites
45-A6191-100MG	100 mg	$1250.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6191-100MG

Properties
Descriptions
Safety Info.
Basic Data

biological source	Streptomyces sp.
form	lyophilized powder
InChI	1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1
InChI key	YAHXZYICKJUJEO-BXLPLHKWSA-N
mode of action	enzyme \| inhibits
potency	1.4 μM K_i
Quality Level	300
SMILES string	Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C
solubility	1-butanol: soluble
	1-propanol: soluble
	DMSO: soluble
	ethanol: soluble
	H₂O: 50 mg/mL
	methanol: soluble
storage temp.	−20°C

Application:	Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis.
Application:	Concentrations for 50% inhibition (μg/ml): papain, 0.16 trypsin, 0.26 cathepsin A, 1.19 cathepsin B, 0.59 cathepsin D, 125 plasmin, >93 chymotrypsin and pepsin, >250 It also has been reported to inhibit calpain I, (porcine) with K_i = 1.4 μM
Biochem/physiol Actions:	Antipain dihydrochloride also inhibits the action of papain and cathespsin B.
Biochem/physiol Actions:	Non-selective serine and cysteine protease inhibitor.
Biochem/physiol Actions:	Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.
General description:	Chemical structure: peptide
Packaging:	1, 5, 25, 100 mg in glass bottle
Preparation Note:	Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.⁸ A stock solution in water or buffer is stable for about a month at -20 °C. Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Preparation Note:	Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352202

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