Azure A chloride
SIGMA/A6270 - Dye content ≥70 %
Synonym: Azure eosin methylene blue; 7-
CAS Number: 531-53-3
Empirical Formula (Hill Notation): C14H14ClN3S
Molecular Weight: 291.80
EC Number: 208-510-7
MDL Number: MFCD00012112
Linear Formula: C14H14ClN3S
Product Type: Chemical
application(s) | diagnostic assay manufacturing hematology histology |
color | green to very dark green |
composition | Dye content, ≥70% |
form | powder or crystals |
InChI | 1S/C14H13N3S.ClH/c1-17(2) |
InChI key | NALREUIWICQLPS-UHFFFAOYSA |
mp | 290 °C (dec.) (lit.) |
Quality Level | 200 |
SMILES string | NC1=CC(SC(C2=N3)=CC(C=C2) |
solubility | water: 1 mg/mL |
storage temp. | room temp |
Application: | Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides. |
Biochem/physiol Actions: | Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain. |
Caution: | Handle and store under argon. Glove bag item. |
Packaging: | 10, 50 g in glass bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 290 °C (dec.) (lit.) |
Storage Temp. | room temp |
Colour Index Number | 52005 |
UNSPSC | 12171500 |