Aprotinin from bovine lung
SIGMA/A6279 - saline solution, 3-7 TIU/mg protein
Synonym: BPTI; Bovine pancreatic trypsin inhibitor; Trasylol; Trypsin inhibitor (basic)
CAS Number: 9087-70-1
Empirical Formula (Hill Notation): C284H432N84O79S7
Molecular Weight: 6511.44
MDL Number: MFCD00130541
Linear Formula: C284H432N84O79S7
Product Type: Chemical
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung. Aprotinin is a single peptide chain with three disulfide bonds. Molecular Weight: ~6511
| biological source | bovine |
| form | saline solution |
| InChI key | ZPNFWUPYTFPOJU-UHFFFAOYSA |
| mol wt | ~6,500 |
| Quality Level | 300  |
| solubility | H2O: soluble >10 mg/mL |
| specific activity | 3-7 TIU/mg protein |
| sterility | aseptically filled |
| storage temp. | 2-8°C |
| UniProt accession no. | P00974  |
| Application: | Aprotinin is largely used as an inhibitor of trypsin. |
| Biochem/physiol Actions: | Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges. |
| Caution: | Aprotinin products should be stored at 2-8°C. |
| General description: | Aprotinin is a 58 amino acid-long protein, with a calculated molecular weight of 6512 Da, that acts as a serine protease inhibitor. Known also as bovine pancreatic trypsin inhibitor (BPTI), aprotinin inhibits serine proteases such as chymotrypsin, kallikrein, plasmin and trypsin. |
| Preparation Note: | This product is an aseptically filled solution in 0.9% NaCl and 0.9% benzyl alcohol. It has an activity of 3-7 TIU/mg protein and 3-21 TIU/mL solution. |
| Unit Definition: | One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-argini |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 3-7 TIU/mg protein |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |