Agarase from Pseudomonas atlantica
SIGMA/A6306 - lyophilized powder, ≥5,000 units/mg protein (Lowry)
Synonym: β-Agarase; Agarose 4-glycanohydrolase
CAS Number: 37288-57-6
MDL Number: MFCD00130365
Product Type: Chemical
Agarase specificity: Agarose is a complex range of polysaccharide chains composed of alternating α-(1-3)-D-galactosyl-β-(1-4)-anhydro-L-galactosyl units.
Enzymatic Assay Colorimetric determination of Agarase activity resulting in reducing suger formation: Agar + H2O Agarase → Reducing Sugar (measured as D-galactose) One unit will produce 1.0 μg of reducing sugar from agar per minute at pH 6.0 at 43 °C.
| biological source | bacterial (Pseudomonas atlantica) |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥5,000 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1000 units in serum bottle |
| Physical form: | Product is formulated with phosphate buffered salts, bovine serum albumin and Agarase. Total protein content ranges between 10 – 30% w/w. |
| Unit Definition: | One unit will produce 1.0 μg of reducing sugar (measured as |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5,000 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.81 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |