Anti-Atg14 antibody produced in rabbit
SIGMA/A6358 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-KIAA0831
Product Type: Chemical
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-ATG14 (Cat. No. A6358). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:500 antibody + ATG14 immunizing peptide (human, 276-288)
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:150-1:300 using whole extracts of mouse 3T3 cells |
| western blot: 1:250-1:500 using whole extracts of human HeLa cells. |
| Application: | Anti-Atg14 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Autophagy Related 14 (Atg14) is an essential gene for autophagy. Atg14 is present on autophagic isolation membranes and is required for autophagosome formation. Atg14 together with vacuolar protein sorting-associated protein 34 (Vps34) and Atg6/Vps30 forms part of a phosphoinositide 3(PI3)-kinase complex in yeast. Two distinct PI3-kinase complexes, complex I and II, were identified. Complex I is involved in autophagy, whereas complex II is involved in vacuolar protein sorting. In mammals, homologs of Vps34, Vps15, Vps30/Atg6, Atg14 and Vps38 were identified as Vps34, p150, Beclin 1, Atg14 and UV radiation resistance associated gene (UVRAG), respectively. Although both Atg14 and UVRAG interact with Beclin 1, they are not present in the same complex. While Atg14 is present on autophagic isolation membranes, UVRAG mainly localized to Ras-related protein Rab9-positive endocytic compartments. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Autophagy-specific subunit of the PtdIns3K complex (Atg14) is encoded by the gene mapped to human chromosome 14q22.3. Atg14, also known as KIAA0831, is an autophagy protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Atg14 recognizes human and mouse Atg14. |
| Storage and Stability: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |