α-Amylase from Bacillus sp.
SIGMA/A6380 - Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)
Synonym: Alpha-Amylase; Glycogenase; 1,4-α-D-Glucan-glucanohydrolase
CAS Number: 9000-90-2
EC Number: 232-565-6
MDL Number: MFCD00081319
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6380-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6380-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6380-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6380-500MG
| application(s) | life science and biopharma |
| assay | ≥30% |
| biological source | bacterial ( |
| form | lyophilized powder |
| mol wt | 50-55 kDa by SDS-PAGE |
| Quality Level | 200  |
| solubility | H2O: soluble 0.1 mg/mL, clear, colorless |
| specific activity | ≥1,500 units/mg protein (biuret) |
| storage temp. | −20°C |
| suitability | suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification |
| technique(s) | SDS-PAGE: suitable |
| type | Type II-A |
| Application: | α-Amylase from Bacillus sp. has been used: • as a component of salivary fluid to perform artificial mastication; in luminal gastrointestinal digestion experiment • as a standard in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine the concentration of α-Amylase • to de-starch the alcohol insoluble residue (AIR) for non-cellulosic neutral monosaccharide analysis |
| Biochem/physiol Actions: | α-Amylase degrades starch into oligosaccharides such as maltose, and glucose and alpha limit dextrin. It hydrolyzes the α-(1→4) glucosidic linkages in polysaccharides of three or more α-(1→4) linked D-glucose units, without hydrolyzing the α-(1→6) bond. It participates in glucose production and is essential for energy acquisition. α-Amylases are widely known industrial enzymes used in the food, detergent, textile, fermentation, and pharmaceutical industries. |
| Features and Benefits: | • α-Amylase from Bacillus licheniformis NCIB 6346 retains over 98% of its activity after 60 minutes at pH 6.2 and 85°C. • Other α-Amylase maintain 100% of their activity after storage for 1 hour at 91°C. |
| Features and Benefits: | Maintains >98% of activity after 60 minutes at pH 6.2 at 85 °C. |
| General description: | α-Amylase, an extracellular enzyme, is present in many animals and plants, and also in microorganisms, such as different Bacillus species. |
| General description: | α-Amylase (1,4-α-d-glucan glucohydrolase), an endo-acting glucanase, is a member of the glycoside hydrolase family 13 (GH13). |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 250, 500 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Preparation Note: | 4× crystallized |
| Preparation Note: | Dissolves in water to form a clear, colorless solution at 0.1 mg/mL concentration. |
| Unit Definition: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥30% |
| activity | specific activity: ≥1,500 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |