Apyrase from potatoes
SIGMA/A6535 - ATPase ≥200 units/mg protein, lyophilized powder
Synonym: Adenosine 5′-diphosphatase; Adenosine 5′-triphosphatase
CAS Number: 9000-95-7
EC Number: 232-569-8
MDL Number: MFCD00130542
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6535-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6535-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6535-200UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6535-2KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6535-500UN
| application(s) | diagnostic assay manufacturing |
| ATPase activity | ≥200 units/mg protein |
| composition | Protein, 25-60% |
| foreign activity | Acid Phosphatase ≤2% of base activity |
| form | lyophilized powder |
| Quality Level | 300  |
| secondary activity | ≥50 % of base activity ADPase |
| solubility | H2O: soluble 1.0 mg/mL |
| storage temp. | −20°C |
| type | Grade VII |
| Application: | Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . |
| Application: | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. |
| Biochem/physiol Actions: | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. |
| Other Notes: | This grade has a low ATPase/ADPase ratio. |
| Packaging: | 100, 200, 500, 1000, 2000 units in glass bottle |
| Packaging: | Sold on the basis of ATPase units. |
| Physical form: | Partially purified, lyophilized powder containing potassium succinate buffer salts. |
| Preparation Note: | Derived from red potato |
| Unit Definition: | One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | ATPase activity: ≥200 units/mg protein; secondary activity: ≥50 % of base activity ADPase |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |