Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep
SIGMA/A6782 - affinity isolated antibody, buffered aqueous solution
Synonym: SAM
MDL Number: MFCD00162644
Product Type: Chemical
antibody form | affinity isolated antibody |
antibody product type | secondary antibodies |
biological source | sheep |
clone | polyclonal |
conjugate | peroxidase conjugate |
form | buffered aqueous solution |
Quality Level | 200 |
shipped in | dry ice |
storage temp. | −20°C |
target post-translational modification | unmodified |
technique(s) | direct ELISA: 1:10,000 |
Application: | Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. Primary T cells isolated from buffy goats were analyzed by western blot using HRP-conjugated sheep anti-mouse IgG as the secondary antibody. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3’,5,5’-tetramethylbenz |
Legal Information: | Ampliflu is a trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Antibody adsorbed with human serum proteins. |
Packaging: | 1 mL in glass bottle |
Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative. |
Preparation Note: | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978). |
Specificity: | Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody reacts with mouse IgG in vitro and in mouse serum and biological fluids. It does not react with human serum proteins. |
Symbol | GHS07,GHS09 |
Signal word | Warning |
Hazard statements | H315 - H317 - H319 - H411 |
Precautionary statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352203 |