β-N-Acetylglucosaminidase from Streptococcus pneumoniae
SIGMA/A6805 - recombinant, expressed in E. coli, buffered aqueous solution
Synonym: β-N-Acetyl-D-hexosaminide N-
CAS Number: 9012-33-3
EC Number: 232-730-2
MDL Number: MFCD00081402
Product Type: Chemical
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| foreign activity | β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.) |
| form | buffered aqueous solution |
| packaging | vial of ≥1.0 unit |
| Quality Level | 100  |
| 200 |
|
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | ≥80 units/mg protein |
| storage temp. | 2-8°C |
| Application: | β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies. |
| Biochem/physiol Actions: | Specific for GlcNAc; not significantly active with GalNAc |
| Biochem/physiol Actions: | This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. |
| Biochem/physiol Actions: | β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β- |
| Physical form: | Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥80 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |